謝淑珠

謝淑珠  (Shu-Chu Shiesh)

醫技系　教授　分機：5764　   E-mail: hsieh@mail.ncku.edu.tw

研究興趣：

1. 肝膽疾病之致病機制與新治療策略之研究：探討膽汁酸在肝膽疾病的致病角色。本實驗室探討重點為：(1)氧化壓力與肝膽疾病之關係。(2)代謝壓力在肝膽疾病所扮演的角色與治療新策略的開發。(3) 新生兒膽汁滯留的致病機轉與治療。

2. 適合體(aptamer)生醫晶片：利用aptamer及微奈米技術的研發，研發晶片檢測生化標誌的臨床應用。

3. 代謝體學的研究：利用LC-MS/MS代謝體技術，開發疾病新標誌、藥物動力學及小分子藥物篩選。

近五年代表作：

1. Chang KW, Li JL, Yang CH, Shiesh SC*, Lee GB*. An integrated microfluidic system for measurement of glycated hemoglobin levels by using an aptamer-antibody assay on magnetic beads. Biosens Bioelectron 2015;68:397-403.
2. Lin HI, Wu CC, Yang CH, Chang KW, Lee GB*, Shiesh SC*. Selection of aptamers specific for glycated hemoglobin and total hemoglobin using on-chip SELEX. Lab Chip 2015;15:486-494.
3. Wu CC, Lin HI, Chang KW, Mai JD, Shiesh SC*, Lee GB*. Measurement of glycated hemoglobin levels using an integrated microfluidic system. Microfluid Nanofluid 2015;18:613-621.
4. Li JL, Chang KW, Wang CH, Yang CH, Shiesh SC*, and Lee GB*. On-chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads. Biosens Bioelectron 2016;79:887-893.
5. Chen PC, Chen YW, Sarangadharan I, Hsu CP, Chen CC, Shiesh SC, Lee GB*, Wang YL*. Field-effect transistor-based biosensors and a portable device for personal healthcare. ECS J Solid State Sc 2017;6:Q71-Q76.
6. Chu CH, Sarangadharan I, Regmi A, Chen YW, Hsu CP, Chang WH, Chyi JI, Chen CC, Shiesh SC, Lee GB* and Wang YL*. Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum. Sci Rep;2017:DOI: 10.1038/s41598-017-05426-6.
7. Kao WC, Chen YW, Chu CH, Chang WH, Shiesh SC, Wang YL, Lee GB. Detection of C-reactive protein on an integrated microfluidic system by utilizing field-effect transistors and aptamers. Biomicrofluidics 2017;11,044105.
8. Yang CH, Chen CY, Chou YY, Chiu HC, Tsai WL, Shiesh SC*. Bile acid profiles in neonatal intrahepatic cholestasis caused by citrin deficiency. Clin Chim Acta 2017;475:28-35.
9. Sarangadharan I, Regmi A, Chen YW, Hsu CP, Chen PC, Chang WH, Lee GY, Chyi JI, Shiesh SC, Lee GB, Wang YL. High sensitivity cardiac troponin I detection in physiological environment using AlGaN/GaN High Electron Mobility Transistor (HEMT) Biosensors. Biosens Bioelectron 2018;100:282-289.
10. Sinha A, Gopinathan P, Chung YD, Lin HY, Li KH, Ma HP, Huang PO, Shiesh SC*, Lee GB*. An integrated microfluidic platform to perform uninterrupted SELEX cycles to screen affinity reagents specific to cardiovascular biomarkers. Biosens Bioelectron 2018;122:104-112.
11. Hung LY, Fu CY, Wang CH, Chuang YJ, Tsai TC, Lo YL, Hsu PH, Chang HY, Shiesh SC, Hsu KF, Lee GB. Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples. Biomicrofluidics 2018;12,054108.
12. Sinha A, Tai TY, Li KH, Gopinathan P, Chung YD, Sarangadharan I, Ma HP, Huang PC, Shiesh SC, Wang YL, Lee GB. An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples. Biosens Bioelectron 2019;129:155-163.
13. Sinhamahapatra A, Gopinnathan P, Chung YD, Shiesh SC, Lee GB. Simultaneous detection of multiple NT-proBNP clinical samples utilizing an aptamer-based sandwich assay on an integrated microfluidic system. Lab Chip 2019;19:1676-1685.
14. Gopinathan P, Sinha A, Chung YD, Shiesh SC, Lee GB. Optimization of an enzyme linked DNA aptamer assay for cardiac troponin I detection: synchronous multiple sample analysis on an integrated microfluidic platform. Analyst 2019;DOI:10.1039/C9AN00779B.
15. Chen YS, Ma YD, Chen CC, Shiesh SC, Lee GB. An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood. Lab Chip 2019; DOI: 10.1039/C9LC00624A
16. Tai TY, Sinha A, Sarangadharan I, Pulikkathodi AK, Wang SL, Lee GY, Chyi JI, Shiesh SC, Lee GB, Wang YL. Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum. Anal Chem 2019;91:5953-5960
17. Chen S, Shiesh SC, Lee GB, Chen CC. Two-step magnetic bead-based (2MBB) techniques for immunocapture of extracellular vesicles and quantification of microRNAs for cardiovascular diseases: A pilot study. PLoS ONE 2020;15:e0229610