首頁師資〉教師指導學生

黃溫雅

黃溫雅 (Wenya Huang, Ph.D.

 

醫學檢驗生物技術研究所教授 分機:5766   Email:whuang@mail.ncku.edu.tw

個人網址:http://mt.ncku.edu.tw/files/11-1371-14490.php#tabs|Teacher:1

專長與研究興趣:

  1. 肝癌發生(hepato-carcinogenesis)與B型肝炎病毒感染之關係與分子機轉: B型肝炎病毒感染是導致肝癌很重要的原因。在癌症發生過程中,細胞常發生基因不穩定性(genomic instability)。因此維持基因的穩定與完整性對於預防癌症發生扮演重要的角色。我們發現B型肝炎病毒的表面抗原pre-S2突變種會造成基因不穩定性及造成DNA損害(DNA damage)。因此我們以B型肝炎病毒為study model,探討慢性B型肝炎帶原者在轉變成肝癌的過程中DNA damage與DNA repair機制的調控及其對HCC發生的影響。
  2. DNA Repair 之分子病理: DNA Repair (DNA修復)是細胞內維持DNA結構完整及修復DNA損害最重要的一個機制。當生物體DNA受到環境途變原(environmental mutagens)例如x-ray,紫外線,以及有毒化學物質等刺激而引起DNA結構不正常時,就需靠DNA Repair 功能來使之恢復正常功能。DNA Repair 功能異常的病人由於DNA突變率較高,導致癌症的機率較正常人高出許多。我們主要的研究方向為探討DNA Repair的分子機轉及其調控,尤其是近年來才分離出的因子hHR23 A及hHR23B (human homolog of Rad23 A / B),探討其在DNA Repair過程中所扮演的角色。除此之外,我們也利用微矩陣(Microarray)生物晶片技術來探討紫外線等環境途變原所引發的DNA Repair分子機轉。同時我們也進一步分析DNA Repair不正常病人的細胞株,以了解DNA Repair異常所引發的相關病理機制。

近五年代表作:

  1. Shen, F.-C., Su, I.-J., Wu, H.-C., Hsieh, Y.-H., Yao, W.-J., Young, K.-C., Chang, T.-C., Hsieh, H.-C., Tsai, H.-N., and Huang, W. (corresponder), 2009. A Pre-S Gene Chip to detect the pre-S deletions in the hepatitis B virus large surface antigen as a predictive marker for hepatoma risk in the chronic HBV carriers. J. Biomed. Sci., 16, 84-91.
  2. Hsieh, Y.-H., Hsu, J.-L., Su, I.-J., and Huang, W. (corresponder), 2011. Genomic instability caused by hepatitis B virus: into hepatoma inferno. Frontiers in Bioscience-Landmark. 17, 2586-2597.
  3. Su, Y.-H., Lee, Y.-L., Chen, S.-F., Hsieh, Y.-H., Hsu, J.-L., Tian, W.-T., Lee, Y.-P., and Huang, W. (corresponder), 2013. Essential role of β-human 8-oxoguanine DNA glycosylase 1 in oxidative mitochondrial DNA repair. Environmental and Molecular Mutagenesis, 54, 54-64.
  4. Hsieh,Y.-H., Su,I.-J., Yen, C.-J., Tsai,T.-F., Tsai, H.-W., Kuo,H.-L., Shen,F.-C., Kao,L.-Y., Huang,Y.-J., Hsieh, W.-C., Tsai,H.-N., and Huang, W. (corresponder), 2013. Histone deacetylase inhibitor suberoylanilide hydroxamic acid suppresses the pro-oncogenic effects induced by hepatitis B virus pre-S2 mutant oncoprotein and represents a potential chemopreventive agent in high-risk chronic HBV patients. Carcinogenesis, 34, 475-485.
  5. Hsu, J.-L., Chuang, W.-J., Su, I.-J., Gui, W.-J., Chang, Y.-Y., Lee, Y.-P., Ai, Y.-L., Chuang, D.-T., and Huang, W. (corresponder), 2013. Zinc-dependent interaction between JAB1 and pre-S2 mutant large surface antigen of hepatitis B virus and its implication in viral hepatocarcinogenesis.J. Virology, 87, 12675-12684.
  6. Hsieh, Y.-H., Su, I.-J., Yen, C.-J., Liu, Y.-R., Liu, R.-J., Hsieh, W.-C., Tsai, H.-W., Wang, L. H.-C., Hsu, C-.C., and Huang, W. (corresponder). Hepatitis B virus pre-S2 mutant surface protein inhibits Nijmegen breakage syndrome 1-mediated DNA repair and induces genome instability. In revision.