首頁師資〉專/兼任師資專長

蔣輯武

蔣輯武 (Chi-Wu Chiang, Ph. D.)                          

分子醫學研究所副教授       分機:3637/ 3591    

E-mail:chiangcw@mail.ncku.edu.tw, pp2acwc@gmail.com

Research interest

磷酸水解酶PP2A在近來已被認為扮演抑制腫瘤形成的角色。PP2A完全酶是由三種次單元所組成,包括了催化次單元C、結構次單元A、及多變的調節次單元B。目前至少有四種B調節次單元家族被發現,而PP2A的多元組合及功能是由於多變的B次單元所造成。我們致力於探討PP2A抑制腫瘤形成的分子機制,也探討有關PP2A的B次單元如何決定PP2A受質特異性及在細胞內的分佈位置及最終所扮演的細胞角色。雖然大部分証據指出PP2A扮演抑癌基因的角色,但是,近來我們發現調節次單元B56γ3在大腸癌的轉移可能扮演促進而非抑制的角色。此外我們也建立了合併雙分子螢光互補作用(BiFC)及螢光共振能量轉移(FRET)的光學影像方法証明了調節次單元B扮演了關鍵角色在導引PP2A座落在細胞中的位置,並且成功的在活細胞中直接觀察PP2A三聚體。

研究方向:

一、探討磷酸酶PP2A在細胞癌化的角色。目前著重在探討帶有B56 gamma3 調節次單元的PP2A在大腸直腸癌中腫瘤轉移的角色及調節細胞癌化的機制。

二、以應用合併雙分子螢光互補作用(BiFC)及螢光共振能量轉移(FRET)方法探究在細胞中PP2A複合體的變化,並藉由此方法來篩選新穎的PP2A複合體的調節分子。

三、針對PP2A與p70S6K之間交互作用的機制來研發癌症新藥。

四、探討PP2A-B55δ在大腸直腸癌中的角色及在腫瘤免疫調節的角色。

近五年代表作:

  1. Tzeng H.T., Li T.H., Tang Y.A., Tsai C.H., Lu F.P.J., Lai W.W., Chiang C.W. and Wang Y.C. (corresponding) Phosphorylation of Rab37 by protein kinase C alpha inhibits the exocytosis function and metastasis suppression activity of Rab37. Oncotarget. 2017 Sep 18;8(65):108556-108570.
  2. Liao S. Y., Chiang C. W., Hsu C. H., Chen Y. T., Jen J., Juan, H. F., Lai W. W., and Wang Y. C.. CK1δ/GSK3β/FBXW7α axis promotes degradation of the ZNF322A oncoprotein to suppress lung cancer progression. Oncogene 2017 Oct 12;36(41):5722-5733.
  3. Lai T. Y., Yen C. J., Tsai H. W., Yang Y. S., Hong W. F., Chiang C. W. (corresponding)The B56γ3 regulatory subunit-containing protein phosphatase 2A outcompetes Akt to regulate p27KIP1 subcellular localization by selectively dephosphorylating phospho-Thr157 of p27KIP1. (2016) Oncotarget 7(4): 4542-4557
  4. Yang C. P., Kuo Y. L., Lee Y. C., Lee K. H., Chiang C. W., Wang J. M., Hsu C. C., Chang W. C., Lin D. Y. RINT-1 interacts with MSP58 within nucleoli and plays a role in ribosomal gene transcription. (2016) Biochem Biophys Res Commun. 478(2):873-80.
  5. Mo S. T., Chiang S. J., Lai T. Y., Cheng Y. L., Cheng-En Chung C. E.,  Kuo S. C. H.,  Reece K. M., Chen Y. C., Chang N. S., Wadzinski B. E., and Chiang C. W. (corresponding) Visualization of subunit interactions and ternary complexes of protein phosphatase 2A in mammalian cells. (2014) PLoS One, 9(12): e116074
  6. Yen C. J., Lin Y. J., Yen C. S., Tsai H. W., Tsai T. F., Chang K. Y., Huang W. C., Lin P. W., Chiang C. W., Chang T. T. (corresponding).Hepatitis B Virus X Protein Upregulates mTOR Signaling through IKKβ to Increase Cell Proliferation and VEGF Production in Hepatocellular Carcinoma. (2012) PLoS One. 2012;7(7):e41931.
  7. Chiang C. W., Liu W. K., Chiang C. W., Chou C. K. (corresponding). Phosphorylation-dependent association of the G4-1/G5PR regulatory subunit with IKKβ negatively modulates NF-κB activation through recruitment of protein phosphatase 5. (2011) Biochem J. 433(1):187-96