首頁師資〉專/兼任師資專長

蔣輯武

蔣輯武 (Chi-Wu Chiang, Ph. D.)                          

分子醫學研究所副教授       分機:3637/ 3591    

E-mail:chiangcw@mail.ncku.edu.tw, pp2acwc@gmail.com

Research interest

磷酸水解酶PP2A在近來已被認為扮演抑制腫瘤形成的角色。PP2A完全酶是由三種次單元所組成,包括了催化次單元C、結構次單元A、及多變的調節次單元B。目前至少有四種B調節次單元家族被發現,而PP2A的多元組合及功能是由於多變的B次單元所造成。我們致力於探討PP2A在抑制腫瘤形成的分子機制,也探討B次單元所扮演的細胞角色。我們建立了合併雙分子螢光互補作用BiFC及螢光共振能量轉移FRET的光學影像實驗方法來探討調節次單元B扮演了關鍵角色在導引完整的PP2A磷酸水解酶座落在細胞中的位置,並且利用BiFC-FRET方法在活細胞中直接觀察PP2A由ABC次單元組合的三聚體。此外,將以前述在活細胞中直接觀察PP2A影像實驗方法為平台,來篩選新穎的調節PP2A的活性的分子或小分子藥物,以做為治療癌症或其他因PP2A失調造成的疾病的新藥。

研究方向:

一、探討磷酸酶PP2A在抑制細胞癌化的機制。此項研究目前著重在探討帶有B56 gamma3 調節次單元的PP2A在大腸、直腸癌中的角色。

二、以應用雙分子螢光互補作用及螢光共振能量轉移方法探究在活細胞中PP2A複合體的變化,並藉由此方法來篩選新穎的PP2A複合體的調節分子或藥物。

三、探討PP2A如何與AMPK交互影響而調節能量新陳代謝、細胞自嗜作用、及腫瘤形成。

近五年代表作:

1. Lai T. Y., Yen C. J., Tsai H. W., Yang Y. S., Hong W. F., Chiang C. W. The B56γ3 regulatory subunit-containing protein phosphatase 2A outcompetes Akt to regulate p27KIP1 subcellular localization by selectively dephosphorylating phospho-Thr157 of p27KIP1. (2016) Oncotarget 7(4):4542-58

2.Yang C. P., Chiang C. W., Chen C. H., Lee Y. C., Wu M. H., Tsou Y. H., Yang Y. S., Chang W. C., Lin D. Y. Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). 2015 J Biomed Sci. 22(1):33-47

3. Mo S. T., Chiang S. J., Lai T. Y., Cheng Y. L., Cheng-En Chung C. E.,  Kuo S. C. H.,  Reece K. M., Chen Y. C., Chang N. S., Wadzinski B. E., and Chiang C. W. (corresponding) Visualization of subunit interactions and ternary complexes of protein phosphatase 2A in mammalian cells. (2014) PLoS One, 9(12): e116074

4. Yen C. J., Lin Y. J., Yen C. S., Tsai H. W., Tsai T. F., Chang K. Y., Huang W. C., Lin P. W., Chiang C. W., Chang T. T. (corresponding).Hepatitis B Virus X Protein Upregulates mTOR Signaling through IKKβ to Increase Cell Proliferation and VEGF Production in Hepatocellular Carcinoma. (2012) PLoS One. 2012;7(7):e41931.

5. Chiang C. W., Liu W. K., Chiang C. W., Chou C. K. (corresponding). Phosphorylation-dependent association of the G4-1/G5PR regulatory subunit with IKKβ negatively modulates NF-κB activation through recruitment of protein phosphatase 5. (2011) Biochem J. 433(1):187-96

6. Hsu C. C., Chiang C. W., Cheng H. C., Chang W. T., Chou C. Y., Tsai H. W., Lee C. T., Wu Z. H., Lee T. Y., Chao A, Chow N. H., Ho C. L.. Identifying LRRC16B as an oncofetal gene with transforming enhancing capability using a combined bioinformatics and experimental approach. (2011) Oncogene. 30 (6):654-667

7. Lee T. Y., Lai T. Y., Lin S. C., Wu C. W., Ni I. F., Yang Y. S., Hung L. Y., Law B. K., and Chiang C. W. (corresponding). The B56γ3 Regulatory Subunit of Protein Phosphatase 2A (PP2A) Regulates S Phase-specific Nuclear Accumulation of PP2A and the G1 to S Transition. (2010) J. Biol. Chem. 285 (28): 21567-21580