首頁師資〉專/兼任師資專長

蔡少正

蔡少正(Tsai, Shaw-Jenq, Ph.D.)

生理學科暨研究所 特聘教授 分機:5426 E-mail: seantsai@mail.ncku.edu.tw

個人網頁:http://phys.med.ncku.edu.tw/files/11-1399-17850.php?Lang=zh-tw

研究興趣

本實驗室主要有三個研究方向。一、缺氧誘導因子(HIF-1α)調控基因與癌症抗藥性之研究。細胞因為缺氧會產生種種不同的生理反應或病理變化,包括學習記憶、癌症、心血管疾病、生長遲緩、腦部疾病、肺部疾病等。我們利用生物資訊學的方法,將基因體中會受缺氧誘導因子調控之基因(包括人類、大鼠、小鼠)找尋出來,並研究其在生理、病理上的功能,尤其著重在癌症抗藥性方面的探討,目前以大腸癌及胰臟癌的抗藥性機制為重點;二、研究整個基因體的調控(Gene regulatory network)及細胞分化、去分化及再分化的機轉。表觀基因體調控(epigenomic modification and reprograming)對細胞之命運影響至巨,甚或造成細胞病變。我們利用生物資訊學、功能性基因體學、系統生物學等尖端方法,對此一現象作深入的研究;三、探討子宮內膜異位(endometriosis)成因之分子機制,子宮內膜異位症約佔生殖年齡期婦女人數的10-15%,大部分會導致不孕症的發生,目前有關子宮內膜異位形成之原因,並不清楚。本實驗室過去幾年的研究,在這個領域已有相當不錯的成果,未來將著重在整合性基因功能上的研究。

近五年代表作

  1. PC Hou, YH Li, SC Lin, SC Lin, JC Lee, PW Lin, JP Liou, JY Chang, CC Kuo, YM Liu, HS Sun, SJ Tsai* Hypoxia-induced downregulation of DUSP-2 phosphatase drives colon cancer stemness. Cancer Res, 77 (16): 4305-4316.
  2. KY Hsiao, YC Lin, SK Gupta, N Chang, L Yen, H. S Sun*, SJ Tsai* Non-coding effects of circular RNA CCDC66 promote colon cancer growth and metastasis. Cancer Res 77:2339-2350. (High cite paper, hot paper)
  3. SC Lin, HC Lee, PC Hou, JL Fu, MH Wu, SJ Tsai* Targeting hypoxia-mediated YAP1 nuclear translocation ameliorates pathogenesis of endometriosis without compromising maternal fertility. J Pathol 242: 476–487. (Cover story)
  4. SC Lin, KY Hsiao, N Chang, PC Hou, SJ Tsai* Loss of dual specificity phosphatase-2 promotes angiogenesis and metastasis via upregulation of interleukin-8 in colon cancer. J Pathol 241 (5): 638-48, DOI: 10.1002/path.4868, 2017
  5. HC Lee, SJ Tsai* Hypoxia: endocrine targets of hypoxia-inducible factors. J Endocrinol, Jul;234(1):R53-R65, 2017.
  6. CW Chien, PC Ho, HC Wu, YL Chang, SC Lin, PW Lin, JC Lee, YJ Chang, HS Sun, SJ Tsai* 2016 Targeting TYRO3 inhibits epithelial-mesenchymal transition and increases drug sensitivity in colon cancer. Oncogene 12(2):61-77. doi: 10.1080/15476278. 2016
  7. SC Lin, CY Kao, HJ Lee, C Creighton, M Ittmann, SJ Tsai, S Tsai, and MJ Tsai Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer. Nat Commun 7:11418
  8. KY Hsiao, MH Wu, N Chang, SH Yang, CW Wu, HS Sun, SJ Tsai* Coordination of AUF1 and miR-148a to destabilize DNA methyltransferase 1 mRNA under hypoxia in endometriosis. Mol Hum Reprod 21:894-904, 2015
  9. SC Lin, YH Li, MH Wu, YF Chang, DK Lee, S Tsai, MJ Tsai, and SJ Tsai* Suppression of COUP-TFII by proinflammatory cytokines contributes to the pathogenesis of endometriosis. J Clin Endocrinol Metab 99(3): E427-37, 2014
  10. TM Chen, YH Shih, JT Tseng, MC Lai, Ch Wu, YH Li, SJ Tsai*, and HS Sun* Overexpression of FGF9 in colon cancer cells is mediated by hypoxia-induced translational activation. Nucleic Acids Res 42:2932-44, 2014
  11. MH Wu, PC Chuang, YJ Lin, SJ Tsai* Suppression of annexin A2 by prostaglandin E2 impairs phagocytic ability of peritoneal macrophage in women with endometriosis. Hum Reprod 28:1045-53, 2013
  12. J Qin, SP Wu, F Dai, X Xie, CM Cheng, C J Creighton, A Frolov, Gustavo Ayala4, X Lin, XH Feng, MM Ittmann, SJ Tsai, MJ Tsai, S Y Tsai   Inhibition of TGF-β-dependent growth barrier by COUP-TFII to promote prostate tumor growth and metastasis. Nature 493: 236-240, 2013