首頁師資〉教師指導學生

橋本昌征

橋本昌征 (Masayuki Hashimoto., Ph.D.)                             

分子醫學研究所 助理教授 分機:3615  E-mail:hashmate@mail.ncku.edu.tw

專長微生物遺傳學、微生物基因體學、細胞生物學、分子生物學

Research and teaching plan

        My major is bacterial genetics, but I also have extensive experiences for bacteriology from molecular level to environmental level. By using the knowledge, I always challenge to ask an interesting question, and try to find out a right answer to the question.

        Escherichia coli is common bacteria, and shows various phenotypes, which are non-pathogenic, enterohemorrhagic, uropathogenic and so on. Such phenotypic features are resulted from different genomic structures. Then, my recent interests are 1) how the pathogenic E. coli are evolved. From our genetic analysis, we found that the restriction enzymes are involved in the genomic evolution to be pathogenic E. coli. Besides, 2) we demonstrated the molecular mechanism to acquire the pathogenic genes, and apply the mechanism to genomic engineering. By using a method developed in our laboratory, we successfully cloned 40kb and 80kb DNA fragment which is too long to be amplified by PCR. We will challenge disease treatments by changing the gut microbiota with engineered E. coli used the method. Through these projects, I want to contribute to synthetic biology.

        My education train your social skills like logical thinking, trouble shooting, even attitude and communication. Research is a good model to learn them, so not only final results, but the processes are also important. The most important skill for master degree is to ask a right question. If you ask wrong question, you cannot get right answer. Furthermore, the most important skill for PhD degree is to ask an interesting question. The interesting question can seek a novel scientific field.

Recent publications

  1. Suparthana IP, Duniaji AS, Hashimoto M. (2018) Investigation to traditionally produced soybean fermented ood (Sere kedele) in Gianyar region-Bali and molecular identification of bacteria involved in their spontaneous fermentation. Scientific Journal of Food Technology. 5 (2) 65-68
  2. Ueda D, Matsugane S, Okamoto W, Hashimoto M, Sato T. (2018) Non-enzymatic pathway with superoxide in intracellular terpenoid synthesis. Angew Chem Int Ed Engl. 57 (32) 10347-10351
  3. Hashimoto M, et al. (2018) Digestion of peptidoglycan near the cross-link is necessary for the growth of Bacillus subtilis. Microbiol. 164 (3) 299-307
  4. Ho YC, et al. (2017) Lrp, a global regulator, regulates the virulence of Vibrio vulnificus. J Biomed Sci. 24 (1) 54.
  5. Pedrolli, D., et al. (2015) The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis. FEBS Journal. 282(16) 3230-42
  6. Kurata, T., et al. (2015) Novel essential gene involved in 16S rRNA processing in Escherichia coli. Journal of Molecular Biology. 427 (4) 955-965.