Hypoxia-inducible mir-210 regulates normoxic gene expression involved in tumor initiation (Mol Cell, 2009, 35:856-867)

報告日期: 2010/03/12
報告時間: 15:10/16:00
報告學生: 簡郡緯
講評老師: 陳炳焜


Hypoxia-inducible mir-210 regulates normoxic gene gxpression involved in tumor initiation


Commentator: Dr. Ben Kuen Chen (陳炳焜老師)

Speaker: Chen Wei Chien(簡郡緯)

Date: 2010.03.12



Hypoxia regulates lots of genes expression through hypoxia inducible factor (HIF). The hypoxia-regulated genes, such as VEGF, GLUT-1 and mircoRNA, play important roles in tumor biology. However, how hypoxia regulates miRNA is still unclear. Herein, the author showed that mir-210 is regulated hypoxia through the binding of HIF-1 on the hypoxia-responsive element of mir-210. By argonaute immunopreciptating, 50 candidated genes were found as potential mir-210 targets and some of these genes were further validated by promoter assay. In functional assay, forced expression of mir-210 inhibited the initiation of tumor growth and the tumor size was negative correlated with mir-210 expression. Moreover, overexpression of mir-210-regulated gene reversed the inhibition of tumor growth by mir-21. Therefore, it suggested mir-210 may function as a tumor suppressor. In conclusion, these data provide the evidence of mir-210 which is regulated by hypoxia in the transcriptional level, and the importance of mir-210 in tumorigenesis. A better understanding of miRNAs in tumorigenesis will open new sight for therapeutic opportunities.



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