Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity (Neuron, 2009, 61:85-100)

報告日期: 2009/05/15
報告時間: 15:10/16:00
報告學生: 王仁伶
講評老師: 吳豐森


Dynamic Microtubules Regulate Dendritic Spine Morphology and Synaptic Plasticity


Neuron (2009) 61:85–100


Speaker: Jen-Ling Wang (王仁伶)

Commentator: Dr. Fong-Sen Wu (吳豐森老師)

Date: 5/15/09  15:10-16:00

Place: Room 602


Dendritic spines contain postsynaptic compartments and play important roles in synaptic plasticity and neural connectivity. It is generally believed that any signals that control spine morphology must be conveyed to the actin cytoskeleton. However, the molecular mechanisms that underlie spine morphological changes are far from complete. The authors exploited microtubule (MT) tip-tracting protein EB3 to elucidate the role of dynamic MTs in dendritic spines. They found that EB3 enter dendritic spines in mature cultured hippocampal neurons to regulate actin dynamics and modulate spine morphology. To elucidate the mechanism that links these MT incursions to regulation of the actin cytoskeleton, the authors reported a novel binding partner of EB3, p140Cap, which is required for spine maintenance. Moreover, p140Cap was shown to interact with cortactin, a Src kinase substrate and F-actin binding protein, capable of promoting actin stability and nucleation. Overexpression of p140Cap or cortactin rescues the EB3 depletion phenotype. The authors proposed that EB3-labeled growing MT ends modulate spine morphology by regulating the localization of p140Cap, thereby altering actin dynamics through the regulation of cortactin. Taken together, this study revealed a novel mechanism for coordinating dynamic MTs with actin cytoskeleton in regulating dendrtic spine morphology and synaptic platicity.