Optogenetic dissection of a behavioural module in the vertebrate spinal cord (Nature, 2009, 461:407-410)

報告日期: 2010/03/23
報告時間: 16:00/16:50
報告學生: 高增婷(英文報告)
講評老師: 許鍾瑜


Optogenetic dissection of a behavioural module in the vertebrate spinal cord

Claire Wyart, Filippo Del Bene, Erica Warp, Ethan K. Scott, Dirk Trauner, Herwig Baier & Ehud Y. Isacoff.  Nature (2009) 461: 407-411.


Student: Tseng-Ting Kao

Commentator: Jung-Yu Hsu

Time: 16:00~16:50

Place: Room 601



Central pattern generators (CPGs) can induce rhythmic and fixative typed movements independent of the peripheral sensory inputs.  In vertebrates, CPGs are activated by the inputs originated from supraspinal glutamatergic synapses or within spinal cord. The authors aimed to identify the spinal neurons triggering the CPG in zebrafish larva using intersectional optogenetics. They used zebrafish line expressing light-gated channel in specific cell types including Gal4s1020t. This transgenic line exhibits a common swim-like behaviour upon the photo-stimulation. The authors found that the activation of Kolmer-Agduhr (KA) cell triggered a locomotion which was similar to the spontaneous forward-swimming. The KA cell is a dendrite-less neuron with undefined functions. It has an ipsilateral axon containing consecutive segments and the protruding cilia into central canal of spinal cord contacting with cerebrospinal fluid. Silencing the activity of KA cells with spinal injection of the GABA inhibitor and with a targeted expression of the tetanus toxin light chain abolished and reduced, respectively, the light-responsive swimming movements. Furthermore, zebrafish larva with the experimental lesion performed in hindbrain still showed the intact swim-like behaviour, suggesting that supraspinal inputs did not involved in KA-elicited swimming movements. They concluded that intra-spinal activation of KA cells only was sufficient to drive the swim-like behaviour.



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2. Scott, E. K. et al. Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nat. Methods 4, 323-326 (2007).