Viral reorganization of the secretory pathway generates distinct organelles for RNA replication (Cell, 2010, 141:799-811)

報告日期: 2010/10/19
報告時間: 16:00/16:50
報告學生: 陳冠儒
講評老師: 王憲威

Full text:

Viral Reorganization of the Secretory Pathway Generates Distinct Organelles for RNA Replication


Nai-Yun Hsu, Olha Ilnytska, Georgiy Belov, Marianita Santiana, Ying-Han Chen, Peter M. Takvorian, Cyrilla Pau, Hilde van der Schaar, Neerja Kaushik-Basu, Tamas Balla, Craig E. Cameron, Ellie Ehrenfeld, Frank J.M. van Kuppeveld and Nihal Altan-Bonnet, Cell 141, 799-811 (2010)


Speaker : Kuan-Ru Chen (陳冠儒)

Commentator: Shainn-Wei Wang Ph.D. (王憲威)

Time: 16:00-16:50

Place: Room 602



In the secretory pathway, intracellular molecules can be transported from ER through Golgi to plasma membrane. Organelle-specific GTPases and specific phosphoinositides participate in intracellular trafficking. Many viruses remodel intracellular membranes and utilize intracellular membranes and cellular protein for replication. Previous studies indicate that Arf1 GTPase and GBF1 (the guanine nucleotide exchange factor of Arf1) are required for enterovirus replication. However, the properties of the replication membranes that are required for viral RNA replication remain unclear. Here this work first demonstrated that enterovirus reorganized the secretory pathway and generate atypical organelles for replication, termed RNA replication organelles. They found viral RNA, viral proteins and the secrecrtory pathway machinery were localized at the RNA replication organelles during enterovirus infection. Besides, enteroviral 3A protein could recruit phosphatidylinositol-4-kinase IIIb (PI4KIIIb) and then generated phosphatidylinositol-4-phosphate (PI4P) lipid-enriched organelles, which promote enteroviral 3D protein binding to PI4P and viral RNA synthesis. Furthermore, they also found that PI4P lipid microenvironment could regulate HCV replication. Thus, this study suggests that positive-strand RNA virus can reorganize the secretory pathway and generate PI4P lipid enrich organelles for RNA synthesis.  



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