A distinctive role for focal adhesion proteins in three-dimensional cell motility (Nat Cell Biol, 2010, 12:598-604)

報告日期: 2011/04/12
報告時間: 16:00/16:50
報告學生: 韓逸成 (英文報告)
講評老師: 吳佳慶

Full Text: http://basicmed.med.ncku.edu.tw/admin/up_img/0412-2.pdf

A Distinctive Role for Focal Adhesion Proteins in Three-Dimensional Cell Motility
Nature Cell Biology. Vol 12, No. 6, June 2010
Speaker: Hans Harn
Commentator: Josh Chia-Ching Wu Ph.D.
Date: April 12th, 2011
Focal adhesions are large multiple-protein assemblies that mediate cell signaling, force transduction and adhesion to the extracellular matrix. Most of the previous studies on focal adhesion were observed at cells in 2D cultures; however, when cells are partially embedded in a 3D matrix, focal adhesions become smaller and their composition differs from the conventional 2D cases1. Furthermore, when the cell is completely buried inside the 3D matrix, focal adhesions are not readily detected2. The role of focal adhesion proteins of a cell in 3D becomes the question. The formation of focal adhesions in 2D, 2.5D and 3D collagen matrix were found different, and vinculin, paxillin, talin, a-actinin, zyxin, VASP, FAK and p130cas do not form aggregates in 3D matrix. Focal adhesion proteins modulate cell motility in both 2D and 3D, but in manners distinct from each other. Protrusion activity and matrix deformation were two processes that modulate cell speed and persistence for cells embedded in matrix. At last, focal adhesion proteins regulated cell speed the same way on glass and on soft substrates in 2D, and this eliminates the compliance differences of matrix in 2 and 3D. In conclusion, modulation of cell speed and persistence on planar substrates by focal adhesion proteins is not predictive of their regulation of cell speed in a matrix, and this highlights the limitations of traditional planar migration studies in understanding 3D cell motility and the role of focal adhesion proteins.
1. Cukierman, E., Pankov, R. & Yamada, K. M. Cell interactions with three-dimensional matrices. Curr. Opin. Cell Biol. 14, 633-639 (2002)
2. Petroll, W. M., Ma, L. & Jester, J. V. Direct correlation of collagen matrix deformation with focal adhesion dynamics in living corneal fibroblasts. J. Cell Sci. 116, 1481-1491 (2003)