Nanoscale architecture of integrin-based cell adhesions (Nature, 2010, 468:580-584)

報告日期: 2011/04/12
報告時間: 17:10/18:00
報告學生: 江宜倫 (英文報告)
講評老師: 鄭宏祺

Full Text:

Nanoscale architecture of integrin-based cell adhesions
Nature, 2010, vol.468, 580-584
Time2011/04/12   PM17:10–18:00     PlaceRoom 602
SpeakerYi-Lun Chiang (江宜倫)
CommentatorHung-Chi Cheng (鄭宏祺教授)
Cellular adhesion is the binding of a cell to a surface, extracellular matrix (ECM) or another cell using cell adhesion molecules which is necessary for morphogenesis, immunity and wound healing. Focal adhesions are specific types of large macromolecular that serve as the mechanical linkages to the ECM, and as a biochemical signaling hub to concentrate and direct numerous signaling proteins at sites of integrin binding and clustering. The complex network of integrins and cytoplasmic proteins forms a below 200-nm plaque. Focal adhesions are widely known about composition, interactions, and dynamics of complex; however, focal adhesion molecular architecture remains unknown. Understanding molecular-scale architecture of cells requires determination of 3D locations of specific proteins with accuracy matching their nanometer-length scale. Existing electron and light microscopy techniques are limited either in molecular specificity or resolution. Authors used 3D super-resolution fluorescence microscopy (interferometric photoactivated localization microscopy, iPALM) to define focal adhesion molecular architecture. Focal adhesion proteins fused with photoactivatable fluorescent proteins (PA-FP) and expressed in human osteosarcoma cells or mouse embryonic fibroblasts (MEFs). The results demonstrated that focal adhesions possess a surprisingly well-organized molecular architecture between integrins and actin that are separated by a about 40-nm focal adhesion core region. The diagonal talin orientation is a role in organizing the focal adhesion strata. The conclusion is that iPALM enables molecular specification and resolution of cellular nanoarchitecturem that provides a molecular blueprint for understanding focal adhesion functions.
1.   Gleb Shtengel, James A. Galbraith, and Clare M. Waterman et al. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. PNAS. 2009; 106(9): 3125-3130.