Plasma fibronectin promotes lung metastasis by contributions to fibrin clots and tumor cell invasion (Cancer Res, 2010, 70(11):4327-4334)

報告日期: 2011/04/15
報告時間: 16:00/16:50
報告學生: 鄭昭旻 (英文報告)
講評老師: 王憶卿
附件下載:

Full Text: http://basicmed.med.ncku.edu.tw/admin/up_img/0415-2.pdf

Plasma Fibronectin Promotes Lung Metastasis by Contributions to Fibrin Clots and Tumor Cell Invasion
 
Gunjan Malik, Lynn M. Knowles, Rajiv Dhir, Shuping Xu, Shuting Yang, Erkki Ruoslahti, and Jan Pilch Cancer Research; 70(11) June 1, 2010
 
Speaker: Chao-Min, Cheng
Commentator: Yi-Ching, Wang
Date/Time: 2011/04/15 16:00-16:50
Room: 602
 
Background:
    Metastasis includes various and complex molecular mechanisms during every stages. Once tumor cells escape the primary site and enter circulation system, the cells flow in bloodstream and adhere to the target organs. The circulating tumor cells are stabilized by thrombus through plasma fibronectin and fibrin in the vasculature. Fibronectin is one of the most important components of clotted plasma, and the upregulation of fibronectin expression is positive correlated to metastasis. Fibronectin has been known to bind to many members of integrin such as αvβ3. Therefore, how fibronectin promotes metastasis by the regulation of integrin is a question.  
Objective
    To dissect the role of plasma fibronectin for metastasis and define the relationship of fibronectin and integrin in the circulating cancer cells.
Results
    From the animal metastasis experiment, the postnatally plasma fibronectin deficient mice revealed significant reduction of lung metastasis, but not liver. They indicated plasma fibronectin is needed for metastasis activity in plasma clot formation. The adhesion and invasion assays showed fibronectin and fibrin can induce cancer cells adhesion and invasion by activated integrin αvβ3.  
Conclusion
    Plasma fibronectin promotes lung metastasis through the activation of integrinαvβ3 at clotted plasma.
 
References
1.     Clark EA, Golub TR, Lander ES, Hynes RO. Genomic analysis of metastasis reveals an essential role for RhoC. Nature 2000;406:5325.
2.     Cheng HC, Abdel-Ghany M, Pauli BU. A novel consensus motif in fibronectin mediates dipeptidyl peptidase IV adhesion and metastasis. J Biol Chem 2003;278:246007.