Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases (PNAS, 2010, 107:4311-4316)

報告日期: 2011/04/19
報告時間: 15:10/16:00
報告學生: 李威廷
講評老師: 張權發
附件下載:

Full Text: http://basicmed.med.ncku.edu.tw/admin/up_img/0419-1.pdf

Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and
MR imaging of proteases
 
PNAS | March 2, 2010 | vol. 107 | no. 9 | 4311–4316
 
Speaker: Wei-Ting Lee (李威廷)
Commentator: Chuan-Fa Chang, Ph.D.
Date: 2011/04/19
Place: Room 602
 
Abstract:
   Bioimaging plays a crucial role in clinical detection and staging of tumor. To increase theefficiency of bioimaging system is an urgent matter in medical science. The authors represented an activatable cell penetrating peptides (ACPPs) technology that consisted of a cationic peptide, an anion peptide, and a cleavable linker between the two different peptides. In the previous study, they showed that ACPPs could be activated by a protease. Once the linker was cleaved by a protease, the cationic CPP is free to carry its cargo into the cells. In this study, the authors demonstrated the in vivo visualization of proteases activities using MRI and fluorescence of dendrimeric nanoparticles (NPs). Dendrimeric NPs that coated with ACPPs showed 4- to 15-fold higher uptake in tumors than unconjugated ACPPs. With the help of fluorescence molecules, tumor could be detected as small as 200 mm and could be analyzed and resected via fluorescenceimagine systems. The MRI detection of gadolinium in the tumor is 30–40 mM. The dosage was useful in T1 contrast and could prolong for 7 days after injection. These results in the paper indicated that ACPPs can improve the quality of bioimaging system and offer a better strategy for treating disease processes.
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Reference:
1.   T. Jiang et al., Proceedings of the National Academy of Sciences of the United States of America 101, 17867 (2004).
2. E. S. Olson et al., Integrative biology: quantitative biosciences from nano to macro 1, 382 (2009).