Cholinergic interneurons control local circuit activity and cocaine conditioning (Science, 2010, 330:1677-1681)

報告日期: 2011/05/06
報告時間: 16:00/16:50
報告學生: 曾文郁 (英文報告)
講評老師: 吳勝男

Full Text:

Cholinergic Interneurons Control Local Circuit Activity and Cocaine Conditioning
Ilana B. Witten, Shih-Chun Lin, Matthew Brodsky, Rohit Prakash, Ilka Diester,
Polina Anikeeva, Viviana Gradinaru, Charu Ramakrishnan, Karl Deisseroth
Science. 2010 ;330:1677-81.
Speaker: Wen-yu, Tzeng(曾文郁)
Commentator: Wu, Sheng-Nan, Ph.D.(吳勝男博士)
Date/Time: 2011-05-06 16:10-17:00
Place: Room 602, College of medicine
There are numerous cholinergic neurons in the central nervous system, and the basic processes by which acetylcholine (Ach) is synthesized, stored and released are the same as in periphery. Previous reports have shown that activation of muscarinic and nicotinic acetylcholine receptors in the nucleus accumbens core is necessary for the acquisition of drug reinforcement. Some studies have shown that cholinergic transmission in the nucleus accumbens (NAc) is required for reward learning. Nonetheless, recent studies have indicated that Ach-eliminated transgenic mice exhibit reliable cocaine-induced conditional place preference (CPP) after conditioning with 5 mg/kg and 10 mg/kg of cocaine. The effects of the cholinergic interneuron on NAc physiology and behavior remain argumentative. Thus, the authors try to examine the net effect of the cholinergic interneurons on NAc-related physiology and behavior. In order to address this question, they take an optogenetic approach by selectively driving or blocking firing in these cells. Their results demonstrate that selectively inhibiting cholinergic interneurons in the NAc has the overall effect of increasing medium spiny neuron (MSN) activity and blocking cocaine conditioning.
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