Immunobiotic Strains Modulate Toll-Like Receptor 3 Agonist Induced Innate Antiviral Immune Response in Human Intestinal Epithelial Cells by Modulating IFN Regulatory Factor 3 and NF-κB Signaling (Front Immunol. 2019, 10:1536.)

報告日期: 2019/10/15
報告時間: 16:00/16:50
報告學生: 李孟佳
講評老師: 凌斌
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Immunobiotic Strains Modulate Toll-Like Receptor 3 Agonist Induced Innate Antiviral Immune Response in Human Intestinal Epithelial Cells by Modulating IFN Regulatory Factor 3 and NF-κB Signaling

Paulraj Kanmani and Hojun Kim

Front Immunol. 2019 Jul 3;10:1536.

Department of Rehabilitation Medicine of Korean Medicine, Dongguk University Ilsan Hospital, Gyeongj-si, South Korea

Speaker: Jeffrey Li (李孟佳)                                    Time: 16:00-16:50, Oct 15, 2019

Commentator: Dr. Pin Ling (凌斌副教授)                   Place: Room 602, College of Medicine

Abstract

    Immunbiotics, microbial strains with positive effect to host immune system, have been reported to reduce the severity of bacterial and viral infections by modulating the host immune responses (ex, antiviral and anti-inflammatory activities). Many researchers have found that probiotics like lactic acid bacteria(LABs) were benefit to host immunity. However, there is still uncertain regarding the detailed mechanisms of the immunoregulatory activity of lactic acid bacteria(LABs). Therefore,the aim of this study was to elucidate whether the specific LABs (L. plantarum DU1, W. cibaria DU1, and L. sakei DU2) modulate toll-like receptor 3 (TLR3) agonist polyinosinic:polycytidylic acid (PolyI:C) induced innate antiviral immune response in human intestinal epithelial cells (IECs). PolyI:C increased the expression of antiviral cytokine interferon-β (IFN-β) and inflammatory cytokines in HCT116 cells. In this study, HCT116 cells were pre-stimulated with LABs isolated from Korean fermented foods, LABs strains were capable to up-regulate antiviral cytokine IFN-β and tight junction proteins (zonula occludens-1 and occludin) but down-regulated inflammatory cytokines (IL-6, IL-8, MCP-1, and IL-1β) in comparison to PolyI: C. In addition, LABs up-regulated the expression of antiviral proteins: 2-5A oligoadenylate synthetase 1 (OSA1), myxovirus resistance protein (MxA), TLR3, and retinoic acid inducible gene-I (RIG-I). The beneficial effects LABs were elucidated by activation of interferon regulatory factor 3 (IRF3) and suppression of nuclear factor-kappa B (NF-κB) pathways. Moreover, LABs increased the expression of negative regulator for NF-κB pathways to suppress the inflammatory responses. Finally, LABs promoted the cell-cell interaction in transwell assay. Labs increased IFN-β, IFN-α, and IL-10 and decreased tumor necrosis factor-α (TNF-α) and IL-1β expression in THP-1 cells that were stimulated by spent medium of HCT-116 cells.In conclusion, LAB beneficially modulate host immune system by enhancing antiviral activity and suppressing inflammatory responses.

Reference

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