Temporal transcriptional profiling of somatic and germ cells reveals biased lineage priming of sexual fate in the fetal mouse gonad (PLoS Genetics, 2012, 8(3):e1002575)

報告日期: 2012/11/20
報告時間: 15:10~16:00
報告學生: 李奕漢
講評老師: 曾大千
附件下載: 下載[1255-1350711791-1.pdf] 

Temporal Transcriptional Profiling of Somatic and Germ Cells Reveals Biased Lineage Priming of Sexual Fate
in the Fetal Mouse Gonad

Jameson SA, Natarajan A, Cool J, DeFalco T, Maatouk DM, Mork L,
Munger SC, Capel B

PLoS Genet. 2012;8(3): e1002575

Speaker: Li, Yi-Han                                    Date: 2012/11/20

Commentator: Tseng, Ta-Chien, Ph.D.                      Place: Room 602

Abstract

In mammals, biological differences between males and females are determined genetically during embryonic development1. It is known that the early gonad is composed of four lineages: supporting cells, interstitial/stromal cells, germ cells, and endothelial cells. Nevertheless, how cells diverge from a common progenitor and adopt specific fates is still poorly understood. The bipotential gonad provides a unique system to study cell fate decisions during mammalian organ development. By examining temporally the transcriptomes of these diverse progenitors as they make their parallel and binary fate decisions, we can understand how cell fate decisions are made in the context of organ development. In this study, the authors analyzed gene expression profiles in the distinct cell lineages of the gonad over the period when sex determination occurs. They conducted microarrays on sorted cells from XX and XY mouse gonads at three time points spanning the period when the gonadal cells transition from sexually undifferentiated progenitors to their respective sex-specific fates. They found that germ cell progenitors were primed with a bias toward the male fate. In contrast, supporting cells were primed with a female bias, indicative of the robust repression program involved in the commitment to XY supporting cell fate. These results confirm and provide an explanation for why the female fate is the developmental default for the gonad, and they advance our understanding of how complex transcriptional networks regulate fate determination during organ development.

 

References:

1.        Eggers, S. & Sinclair, A. Mammalian sex determination-insights from humans and mice. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 20, 215-238 (2012).