Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women (Nat Med, 2012, 18:413-421)

報告日期: 2012/11/20
報告時間: 16:00~16:50
報告學生: 馬文輝(英文報告)
講評老師: 楊尚訓
附件下載: 下載[1256-1350711842-1.pdf] 

 

Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women
White Y, Woods D, Takai Y , et al.

(Nat Med. 2012 Mar; 18(3):413-21 )

Speaker: Wen-Hui Ma                         Commentator: Shang-Hsun Yang,Ph.D.

Time: 11/20 /2012 16:1017:00                 Place: Room 602

Abstract

Based on the previous theory in 1950s, women could only produce limited oocytes during the reproductive period after birth because limited oogonia are stored inside ovaries. However, this fundamental theory was challenged in 2004 due to the identification of oocyte-producing stem cells (OSCs) in ovaries. These OSCs could propagate through self-renew, but whether these cells could produce functional oocytes were still unknown. In this paper, the authors isolated ovarian stem cells from human ovarian tissue though the fluorescence-activated cell sorting protocol using DDX4, a unique protein on the surface of the stem cells. These cells could be expanded in vitro and spontaneously transformed into oocytes, which were characterized by morphology, gene expression and haploid status.To examine the functional capabilities of the stem cells, the investigators injected GFP-labeled mouse OSCs into the immunodeficient mice,several months later, the GFP-labeled mouse oocytes were flushed from the oviducts successfully fertilized in vitro and developed to the blastocyst stage. To confirm oogenesis in human OSC model, the formation of follicles containing GFP-positive oocytes was found after one to two weeks of human OSCs xenotransplantation into immunodeficient female mice, implying the normal and health oocytes were generated. These studies supported not only the existence of OSCs, but also the capacity of these OSCs processing the oogenesis to generate fertilizable oocytes. These results provide the insight for potential application in infertile patients.

 

References

1.         Johnson, J., Canning, J., Kaneko, T., Pru, J. K., and Tilly, J. L. (2004). Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature 428, 145-150.

2.         Zou, K., Yuan, Z., Yang, Z., Luo, H., Sun, K., Zhou, L., Xiang, J., Shi, L., Yu, Q., Zhang, Y., et al. (2009). Production of offspring from a germline stem cell line derived from neonatal ovaries. Nat Cell Biol 11, 631-636.