KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum. (Cell Rep pii: S2211-1247(15)00167-9, 2015)

報告日期: 2015/10/13
報告時間: 3:10/4:00
報告學生: Amrita VATS(英文報告)
講評老師: 林以行
附件下載: 下載[1499-1443053354-1.pdf] 

KDELR Receptors Assist Dengue virus Exit from the Endoplasmic Reticulum

Ming Yuan Li,Marc Grandadam,Kevin Kwok,Thibault Lagache,Yu Lam Siu,Jing Shu Zhang,Kouxiong Sayteng,Mateusz Kudelko,Cheng Feng Qin,Jean-Christophe Olivo-Marin,Roberto Bruzzone,and Pei Gang Wang.

Speaker: Amrita vats                  Commentator: Prof Yee Shin Lin

Time: 3:10- 4:00 PM                   Room : 602

Membrane receptors at the surface of target cells are key host factors for virion entry; however, it is unknown whether trafficking and secretion of progeny virus requires host intracellular receptors. In this study ,the author has demonstrated that dengue virus (DENV) interacts with KDEL receptors (KDELR) ,which cycle between the ER to Golgi apparatus ,for vesicular transport from ER to Golgi . Depletion of KDELR by Si RNA reduced egress of both DENV progeny and recombinant sub viral particles (RSPs). Coimmunoprecipitation of KDELR with dengue structural protein PrM required three positively charged residues at the N –terminus ,whose mutation disrupted protein interaction and inhibited RSP transport from the ER to Golgi .Finally ,siRNA depletion of class II Arfs ,which results in KDELR accumulation in the Golgi ,phenocopied results obtained with mutagenized PrME and KDELR knockdown .The findings in the work have uncovered a function for KDELR as an internal receptor involved in DENV trafficking.

REFERENCES:

  1. Class II ADP ribosylation factors are required for efficient secretion of Dengue viruses.Mateusz Kudelko et al. Journal of Biological chemistry. 2012, Vol 287.
  2. Efficient assembly and secretion of recombinant sub viral particles of the four dengue serotypes using native PrM and E proteins. Pei-Gang Wang et al.Plos One .2009.Vol 4.