G3BP1, G3BP2 and CAPRIN1 Are Required for translation of Interferon Stimulated mRNAs and Are Targeted by a Dengue Virus Non-coding RNA (PLOS Pathogens 2014, 10(7):e1004242)

報告日期: 2014/10/14
報告時間: 3:10/4:00
報告學生: Nurhafiza Binti Zainal
講評老師: 彭貴春
附件下載: 下載[1429-1412164061-1.pdf] 

G3BP1, G3BP2 and CAPRIN1 Are Required for Translation of Interferon Stimulated mRNAs and Are Targeted by a Dengue Virus Non-coding RNA

Katell Bidet et al. PLoS Pathogens (2014) 10: e1004242

Speaker: Nurhafiza Zainal                                    Time: 15:10~16:00, Oct 14, 2014

Commentator: Prof. Oscar Perng                              Venue: Room 602

 

Abstract

Dengue is a systemic viral infection transmitted between humans by Aedes mosquitoes and it causes symptoms ranging from mild febrile illness to severe disease. Over 3.6 billion people globally are at risk for dengue virus (DENV) infection, with an estimated 390 million infections annually1. There is no specific treatment or approved vaccine for DENV infection. The pathogenesis of dengue has been extensively investigated, although it is not completely umderstood. The host interferon (IFN) response is one of the most powerful components in the innate immune system and is known to have a role in detecting and clearing a wide range of viral infections2. However, the mechanism on how DENV subverts the host IFN system during infection remains unclear and controversial. In this study, the authors identified three conserved host RNA-binding proteins (RBPs) G3BP1, G3BP2 and CAPRIN1 as novel regulators of the IFN response against DENV-2. These RBPs are essential for protein accumulation of several interferon stimulated genes (ISGs) and efficient translation of PKR and IFITM2 mRNAs. In addition, antiviral activity was found to be antagonized by DENV-2 non-coding subgenomic flaviviral RNA (sfRNA) by binding to G3BP1, G3BP2 and CAPRIN1, which led to significant reduction of ISG mRNA translation. This finding provides an important guide to understanding regulation of interferon stimulated gene expression, as well as the diversity of strategies used by DENV to counteract the host interferon system.

 

References:

1)     Bhatt, S. et al. (2013) The global distribution and burden of dengue. Nature 496:504-507

 

2)     Garcia-Sastre, A. & Biron, C. A. (2006) Type 1 interferons and the virus-host relationship: a lesson in detente. Science 312: 879–882.