Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis (cell,2014,158, 1110–1122)

報告日期: 2015/04/10
報告時間: 17:10/18:00
報告學生: 鄭立欣
講評老師: 王憶卿
附件下載: 下載[1482-1425858905-1.pdf] 

Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis

Speaker:Lin-Hsin Cheng                                       Date:2015/04/10

Commentator: Dr.Yi-Ching Wang                           Room:602

Abstract:

Metastasis is a major cause of mortality for years. Circulating tumour cells (CTCs) are present in the blood of patients with cancer but the dedication tometastasis is not well described. In the circulation, CTCs have been detected either presenting as single cells or clusters. The authors demonstrate that CTC clusters have a higher ability to form lung metastases than single CTCs. The authors also use CTC-I Chip to collect single CTCs and CTC clusters from the patients with metastatic lesions. RNA sequencing of both single and clustered CTCs from breast cancer patients identified several genes expressed at elevated levels in CTC clusters, one of which – a protein called plakoglobin.plakoglobin is a component of desmosomes as adhesive proteinlocalized at the plasma membrane. The authors also find that plakoglobin might keep their CTC clusters together while the initiation of primary tumor cell clumps. In addition, it is found that plakoglobin knockdown do not affect growth of the primary tumour or the number of single CTCs in the circulation, but it do reduce the numbers of CTC clusters and metastatic foci in the lungs. These data suggest that rare CTC clusters are the most likely initiators of metastatic lesions. Furthermore, it might be a good strategy targeting CTC clusters to prevent metastasis in the future.

References:

  1. Nicola Aceto et al. (2014). Cell 158, 1110-22.
  2. Yu et al. (2011). J Cell Biol 192, 373–82.