Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain (Nature Communications 2016, 7:11100, Dio:10.1038)

報告日期: 2016/11/25
報告時間: 4:00/4:50
報告學生: 余亭萱
講評老師: 曾淑芬
附件下載: 下載[1579-1473666629-1.pdf] 

Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain

Authors: Hiromu Monai, Masamichi Ohkura, Mika Tanaka, Yuki Oe, Ayumu Konno, Hirokazu Hirai, Katsuhiko Mikoshiba, Shigeyoshi Itohara, Junichi Nakai, Youichi Iwai and Hajime Hirase Journal: Nat. Commun. 7: 11100 (2016).


Speaker: Ting-Hsuan Yu (余亭萱)                         Date: 11/25/2016

Commentator: Shu-Fen Tzeng (曾淑芬) 老師               Room: Room 602


Transcranial direct current stimulation (tDCS) is a non-invasive field stimulation. It is thought to be a potential therapy on numerous neurological conditions such as depression. tDCS is also known to enhance memory. In previous studies, tDCS increases the excitability of the motor cortex in human subjects and DCS enhances synaptic response that depends on the N-methyl-D-aspartate receptor (NMDAR) activation on mouse motor cortical slices. However, the detail mechanism of tDCS in vivo still remain unknown. It is considered that astrocytes play an important role in NMDAR-dependent plasticity induced by gliotransmission which intracellular Ca2+ elevation involved. Therefore, the authors used a transgenic mouse expressing G-CaMP7 in astrocytes to monitor the dynamic neuronal activity of the brain during tDCSby Ca2+ imaging. They found that tDCS produced large and synchronized Ca2+ surges in astrocytes, whereas neurons did not show obvious activity changes. Both administration of prazosin, an inverse agonist at α1 adrenergic receptors (A1AR), and inositol trisphosphate receptor type 2 (IP3R2) knockout mice, astrocytic Ca2+ surges are absent. In addition, tDCS enhanced visual flash evoked potential. Suggesting thattDCS-induced sensory plasticity depends on A1AR and IP3R2. These results suggest that tDCS changes the plasticity of the cortex through astrocytic Ca2+/IP3 signaling.



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