CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis (Current Biology 2016, 26:1127–1137)

報告日期: 2016/11/22
報告時間: 3:10/4:00
報告學生: 許志祥
講評老師: 洪良宜
附件下載: 下載[1600-1473728212-1.pdf] 

CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis

Sihem Zitouni,Maria E. Francia,Filipe Leal,Susana Montenegro Gouveia,Catarina Nabais,Paulo Duarte,Samuel Gilberto,Daniela Brito,Tyler Moyer,Steffi Kandels-Lewis,Midori Ohta,Daiju Kitagawa,Andrew J. Holland,Eric Karsenti,Thierry Lorca,Mariana Lince-Faria,and Mo´ nica Bettencourt-Dias

Current Biology 26, 1127–1137 

Speaker:Jhih-Siang Syu   Commentator: Prof. Liang-Yi Hung

Date: 2016/11/22

Abstract:

Centrosome is composed of centrioles and pericentriolar material (PCM). Centrioles are involved in cell division, cellular organization, ciliogenesis and development. Centriole biogenesis which produces new centriole occurs only once in each cell cycle and regulates cell-cycle progression by formatting right numbers at right time. The initiation of centriole biogenesis is dependent on polo-like kinase 4 (PLK4) which is recruited to mother centriole. Then PLK4 recruits STIL (SCL/TAL1/SAS5/Ana2) to centriole and phosphorylates STIL. After that, phosphorylated STIL brings SAS6 to the centrioles for formation of the centriole cartwheel which is a structure in early S phase. In addition, PLK4, STL and SAS6 are located in centrosome from mid-G1 to the end of mitosis. However, the regulation of centriole biogenesis is unclear. Here they discover that in the mitosis, CDK1-Cyclin B complex binds STIL and inhibits the phosphorylation by PLK4. CDK1-Cyclin B complex releases STIL and forms PLK4-STIL complex in G1 phase to regulate centriole biogenesis. In addition, inhibiting the activity of CDK1-Cyclin B complex leads to form PLK4-STIL complex and phosphorylate STIL in mitosis. Furthermore, they show that CDK1-Cyclin B complex and PLK4 bind on the same region of STIL. Finally, they discovered that CDK1-CyclinB prevents STIL-PLK4 interaction by binding STIL through the same region as PLK4 to regulate centriole biogenesis.