Lab-on-a-Membrane Foldable Devices for Duplex Drop-Volume Electrochemical Biosensing Using Quantum Dot Tags (Anal. Chem. 2016, 88:6897−6904)

報告日期: 2016/11/29
報告時間: 3:10/4:00
報告學生: 陳宏銘
講評老師: 謝達斌
附件下載: 下載[1603-1473728415-1.pdf] 

Lab-on-a-Membrane Foldable Devices for Duplex Drop-Volume Electrochemical Biosensing Using Quantum Dot Tags

Christos K, Michailia A, Anastasios E, et al.

Anal. Chem. 2016, 88, 6897−6904

Speaker: Hong-Ming Chen

Commentator: Dar-Bin Shieh

Date: 2016/11/29, 15:10-17:00

Location: Room 602

Abstract

Food adulteration is a very important issue in recent year for people with considerable emphasis on food label and healthy dietary. In order to facilitate on-site analysis, this study developed a set of on-site analysis device to identify the goat milk adulteration. “Goat milk adulteration with bovine milk can be detect by bovine Casein and bovine IgG” This device is based on electrochemistry to detection the current changes with different quantum dots. This device can detect two analytes in a single analytical run. It uses nylon substrate, and prints two circular zone by dielectric ink. Between two circular zones, three electrodes (the working electrode, the reference electrode and the counter electrode) are fabricated. To enhance the sensitivity, Bi (bismuth) film consisted of Bi nanoparticles are coated on the working electrode. The detected targets, bovine Casein and bovine IgG, are immobilized separately in the two analytic circular zones, and then biotinylated Ab was added. Streptavidin-conjugated Pb-and Cd-based quantum dots (QDs) were added for detection. Pb(II) and Cd (II) were released in the two analytic zones by acid dissociation. Folding the two analytic zones to contact with the working electrode coated a bismuth layer results in the potential changes. It can be detected by the anodic stripping voltammetric method. This results showed that the limits of detection (LOD) of bovine casein (CN) and bovine IgG (bIgG) in goat milk were 0.04 μg/mL and 0.02 μg/mL, respectively. The working ranges of bovine Casein and bovine IgG in goat milk were 1% and 50% (V/V) in the results of evaluation of goat milk adulteration with bovine milk. Thus, this portable device can indeed assist people on-site analysis to detect goat milk adulteration with bovine milk. Therefore, this on-site analytical device provide us a new direction of immunoassay for diagnosis which is more convenient, simple, and easy to be used in the future.

Reference

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