Identification of bronchioalveolar stem cells in normal lung and lung cancer (Cell, 121:823-835, 17 June 2005)

報告日期: 2005/09/23
報告時間: 15:10/16:00
報告學生: 林佩儒
講評老師: 吳昭良
附件下載:

Identification of Bronchioalveolar Stem Cells

in Normal Lung and Lung Cancer

Cell 121: 823-835, 2005.

 

Speaker: 林佩儒

Time: Sep. 23, 2005, 15:10-16:00

Commentator: 吳昭良 教授

Place: Room 602

Abstract:

        Stem cells are believed to play roles in tissue homeostasis and tumorigenesis. Several stem cell niches which maintain the pulmonary epithelium have been identified in pollutant-induced lung injury models. A cell population named double positive cells (DPCs) has been identified, which express alveolar-specific prosurfactant apoprotein-C (SP-C) marker and Clara cell antigen (CCA). Furthermore, it has been shown that pleuripotential tumor cells exist in Lox-K-ras G12D conditional mouse lung tumor. In this study, the authors demonstrated the existence of counterparts of DPCs, which were located at bronchioalveolar duct junction (BADJ) in normal adult lung. The cell population responded to naphthalene/bleomycin- induced bronchiolar/alveolar damage and played a role in lung-tissue homeostasis. To verify their extensive self-renewal and multilineage differentiation potential in vitro, DPCs were isolated from mouse lung through FACS-sorted scheme (Sca-1+/CD45-/Pecam-/CD34+), and designated bronchioalveolar stem cells (BASCs). In Lox-K-ras mice, BASCs expansion was observed in the tumorigenic lesions and subsequently differentiated toward the alveolar lineage. Combination of naphthalene administration in animal models showed higher tumor number and burden, suggesting the role of BASCs on tumor initiation. These results implicated that BASCs with stem-cell like features participated in the transformation of normal lung epithelium into adenocarcinoma. Moreover, it also provides new insights for the development of therapeutic strategy for lung cancer or genetic diseases affecting the lung.

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