Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein (The EMBO J., 24:2827-2838, 2005

報告日期: 2005/09/27
報告時間: 16:00/16:50
報告學生: 鄒志成
講評老師: 何漣漪
附件下載:

GP96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

                                              The EMBO Journal (2005) 24, 2827–2838

Speaker鄒志成

Commentator何漣漪  老師

Time16:00-17:00  9/27/2005  

 

Listeria monocytogenes is a ubiquitous food-borne gram-positive bacterium, responsible for life-threatening infections in humans and animals. It is a facultatively intracellular pathogen that is able to enter and multiply in both professional and nonprofessional phagocytes such as epithelial cells or hepatocytes. The interaction of L. monocytogenes with host cells is a key event in the pathogenesis of listeriosis, a process that involves a number of surface proteins. Surface proteins known to be attached by a covalent linkage to the peptidoglycan (PG) were the LPXTG proteins. By comparative genomic, Vip, one of the LPXTG surface proteins was identified as absent from the nonpathogenic species L. innocua. RT-PCR and Northern blot analysis showed that vip gene was positively regulated by PrfA. In this paper, the authors demonstrated that Vip is a surface protein anchored to the bacterial cell wall by sortase A and is required for entry into eukaryotic cell. Furthermore, Gp96, the surface cellular receptor of Vip was identified by ligand overlay approach. In vivo showed that bacterial counts for vip mutant were significantly affected as compared to the wild-type strain in all organs. Therefore, Vip appears as a new virulence factor and plays an important role in L. monocytogenes pathogenesis.

References

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