Suppression of microRNA-silencing pathway by HIV-1 during virus replication (Science, 2007, 315:1579-1582)

報告日期: 2007/10/30
報告時間: 16:00/16:50
報告學生: 鄭 烈
講評老師: 楊孔嘉

Suppression of MicroRNA-Silencing Pathway by HIV-1 During Virus Replication

Kevin Lebrigand, Bruno Cardinaud, Thomas Maurin, Pascal Barbry, Vincent Baillat,

Jacques Reynes, Pierre Corbeau, Kuan-Teh Jeang, Monsef Benkirane

Science, 2007, (315): 1579-1582

Presenter: Lie Cheng

Commentator: Kung-Chia Young. Ph.D

Presentation date: Oct 30, 2007

Room 601 of medical college


microRNA (miRNA) is one of the RNA silencing tools. It’s a short single-stranded non-coding RNA (about 21 to 23 oligonucleotides) and often transcribed by RNA polymerase II. The miRNA regulates the expression of target gene in specific sequence manner via translational inhibition or targeting the mRNA to degradation. In addition, accumulating evidence suggests that the miRNA pathway also controls the replications of both RNA and DNA viruses. To investigate whether the regulation of the miRNA-silencing pathway may influence the replication of HIV-1 virus or not, the authors suppress type III RNase Dicer and Dorsha, which involved in miRNA silencing machinery, in HIV-1 infected PBMCs. The suppression leads to the HIV replication in more efficient manner. In turn, the replication of HIV-1 can also be suppressed when cell treated with miR-17/92, a polycistronic miRNA cluster, via downregulating the expression of a Tat transactivator histone acetylase PCAF through PCAF/Tat pathway. This study verifies the correlation between the cell miRNA machinery and the replication of virus in the HIV invading cell, and that may be helpful in future HIV therapy.