Activation of NF-B by the intracellular expression of NF-B-inducing kinase acts as a powerful vaccine adjuvant (PNAS, 2006, 93:14459-14464)

報告日期: 2007/10/02
報告時間: 17:10/18:00
報告學生: 吳妙文
講評老師: 余俊強
附件下載:

Activation of NF-kB by the intracellular expression of NF-kB-inducing kinase acts as a powerful vaccine adjuvant

 

Speaker: Miao-Wen Wu 

Commentator: Dr. Chun-Keung Yu

Date: 2007/ 10/ 02

Place: Room 602

Abstract

Successful vaccines have been generated largely against viruses that cause acute self-limiting infections followed by long-lasting immunity so for. These vaccines contain attenuated or inactivated whole organisms to elicit neutralizing antibodies. However, pathogens causing chronic infection such as human immunodeficiency virus (HIV), hepatitis C virus (HCV), tuberculosis (TB), influenza, or in the case of the cancer and so on are still no effective vaccines for theses emerging global infectious disease and the natural immune response is insufficient for protection. Therefore, it is important to develop a new strategy for improving and generating effective vaccines. In this paper, the author proposed an approach for enhancing vaccine immunogenicity by the activation of intracellular expression of NF-kB-inducing kinase (NIK) acts as a powerful vaccine adjuvant in order to generation of adaptive immune response and adaptive immunity. The author used T cell proliferation Assays, Western blot, EMSA, FACS, LNC proliferation and CTL assays to prove NIK can be a potential vaccine adjuvant. The results indicate that NIK enhances DC antigen-presenting in both allogeneic and antigen-specific T cell proliferation in vitro and also NIK enhances antigen-specific antibody response against a vector-encoded antigen in vivo as well as shift them toward a Th1 type. On the other hand, NIK-induced GFP-specific antibody responses are long-lived and can be boosted. Finally, NIK enhances antigen-specific cell-mediated immune responses. In a word, this potential approach of inducers of NF-kB activation by NIK provides a powerful tool for developing genetic vaccine.

References

1.     Andreakos E., Williams R. O., et al., “Activation of NF-kB by the intracellular expression of NF-kB-inducing kinase acts as a powerful vaccine adjuvant”, PNAS 103 (93), 14459-14464, (2006).

2.     Andreakos E., et al, “Distinct pathways of LPS-induced NF-kB activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP., Blood 103, 2229-2237, (2004).