A functional screen implicates microRNA-138-depednent regulation of the decalmitoylation enzyme ATP1 in dendritic spine morphogenesis (Nat Cell Biol, 2009, 11:705-716)

報告日期: 2009/12/04
報告時間: 15:10/16:00
報告學生: 陳建仲
講評老師: 黃阿敏
附件下載:

http://basicmed.med.ncku.edu.tw/admin/up_img/981204-1.pdf

A functional screen implicates microRNA-138-dependent regulation of the depalmitoylation enzyme APT1 in dendritic spine morphogenesis

Nature cell biology 11:705-716, 2009

 

Speaker: Chien-Chung Chen ( 陳建仲 同學 )

Commentator: A-min Huang Ph. D. ( 黃阿敏 老師 )

Time: 15:00 ~ 16:00 December 4, 2009

Place: Room 602

Abstract

Dendritic spine is a specific structure which needs a rapid regulation of protein expression to affect transmitter release. The regulation of spine volume or density contributes to the activity of neuronal circuit. Some specific mRNA located in the spine for an immediate requirement to save the time that protein synthesized from cell body. MicroRNA (miRNA) inhibits the expression of mRNA by targeting to specific site in the 3’UTR of mRNA, thus result in gene silencing. In the mammalian nervous system, miRNAs plays a key role during cell specification, neurite outgrouth and spine development. To address whether the spine specific miRNA target included in the signaling pathway of spine morphogenesis, the author demonstrated that synaptically enriched miRNA, miR-138, negatively regulates the size of dendritic spines in hippocampal neuron. Acyl protein thiosterase 1 (APT1), an enzyme regulate the palmitoylation status of proteins, interfered by miR-138 to suppress spine enlargement. The same situation could also present when APT1 knock down and the substrate, a13 subunit of G protein, was blocked. This finding define a previously unknown mechanism by which miRNAs control dendritic spine morphogenesis and indicate a unrecognized complexity of miRNA function in the regulation of synaptic plasticity in mammalian neurons.  

 

References

Schratt, G. M. et al (2006). A brain-specific microRNA regulates dendritic spine development. Nature.439:283–289.

Mette, C. and Gerhard, M. S. (2009). MicroRNA involvement in developmental and functional aspects of the nervous system and in neurological diseases. Neuroscience letters. 466:55-62