A small molecule enhances RNA interference and promotes microRNA processing (Nat Biotechnol, 2008, 26:933-940)

報告日期: 2008/11/18
報告時間: 16:00/16:50
報告學生: 林君杰
講評老師: 劉校生
附件下載:

http://basicmed.med.ncku.edu.tw/admin/up_img/971118-2.pdf

A small molecule enhances RNA interference and promotes microRNA processing

Ge Shan, Yujing Li, Junliang Zhang, Wendi Li, Keith E Szulwach, Ranhui Duan, Mohammad A Faghihi, Ahmad M Khalil, Lianghua Lu, Zain Paroo, Anthony W S Chan, Zhangjie Shi, Qinghua Liu, ClaesWahlestedt, Chuan He & Peng Jin

 

Nature Biotechnology, Volume 26, Number 8, Aug 2008

 

SpeakerChun-Chieh Lin

CommentatorDr. Hsiao-Sheng Liu

Date : 18 November, 2008 (16:00~16:50)

Place : Room 602

 

Abstract

   RNAi pathway is one of the most important regulatory pathways in eukaryotic cells. It can modulate cell development, growth, death and stem cell differentiation by interfering mRNA to repress gene expression. To get more details about RNAi pathway, a small molecule called enoxacin which can increase RNAi processing has been chosen. In this study, enoxacin could facilitate the maturation and loading of siRNAs but did not increase the expression of RNAi-associated proteins. By performing in vitro miRNA precursor processing assay and RNA binding assay, enoxacin increased the binding affinity of TRBP for miRNA precursors and enhanced the processing of miRNAs by Dicer and TRBP together. Besides in vitro, enoxacin also enhanced siRNA processing activity in mouse model. Taken together, small molecules with enhancing RNAi pathway can be potential research and therapeutic tools.

Reference

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2.     Running interference: prospects and obstacles to using small interfering RNAs as small molecule drugs. Annu. Rev. Biomed. Eng. 8, 377–402 (2006).