miR-145 and miR-143 regulate smooth muscle cell fate and plasticity (Nature, 2009, 460:705-710)

報告日期: 2009/12/08
報告時間: 16:00/16:50
報告學生: 嚴佳冠
講評老師: 吳梨華


miR-145 and miR-143 regulate smooth muscle cell fate and plasticity

Kimberly R. Cordes, Neil T. Sheehy, Mark P. White, Emily C. Berry, Sarah U. Morton,Alecia N. Muth, Ting-Hein Lee, Joseph M. Miano, Kathryn N. Ivey

& Deepak Srivastava.


Nature, August 2009 vol. 460

Date:2009 12/08

Speaker: chia-kuang Yen

Commentator: Li-Wha Wu



MicroRNAs represent a class of small (,20–25 nucleotides), non-coding RNAs that are key regulators of many cellular events, includ-ing the balance between proliferation and differentiation during tumorigenesis and organ development. miR-143 is highly conserved and lies within1.7 kilobases (kb) of another conserved miRNA, miR-145, on mousechromosome 18. Both miRNAs are down-regulated in various cancer cell lines, colon cancers, and lung cancers. This paper show that miR-145 and miR-143 are co-transcribed inmultipotent murine cardiac progenitors before becoming localized to smooth muscle cells, including neural crest stem-cell-derived vascular smooth muscle cells. Furthermore, miR-145 and miR-143 cooperatively targeted a network of transcription factors, including Klf4(Kruppel-like factor 4),myocardin and Elk-1 (ELK1,member of ETS oncogene family), to promote differentiation and repress proliferation of smooth muscle cells. These findings demonstrate that miR-145 can direct the smooth muscle fate and that miR-145 and miR-143 function to regulate the quiescent versus proliferative phenotype of smooth muscle cells.



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