A traffic-activated Golgi-based signalling circuit coordinates the secretory pathway (Nat Cell Biol, 2008, 10:912-922)

報告日期: 2008/11/25
報告時間: 16:00/16:50
報告學生: 劉相君(英文報告)
講評老師: 林秋烽


A traffic-activated Golgi-based signalling circuit coordinates the secretory pathway

Nature Cell Biology. 2008, 10: 912- 922


Speaker: Hsiang-Chun Liu

Commentator: Dr. Chiou-Feng Lin



The molecular basis of coordination for secretory pathway, biosynthesized materials move from the endoplasmic reticulum (ER) to the Golgi apparatus in antegrade (forward) transport or retrograde (retrieval) transport machinery proteins from Golgi to ER is still unclear. Several autoregulatory mechanisms, including vesicle budding and pH-dependent protein trafficking, might contribute to the transport between two compartments. However, the authors examined a specific model depending on signalling circuits involved in trafficking. In the beginning, They demonstrated that traffic pulse indeed induce signaling of Tyr phosphorylation on Golgi complex and most signalling cascades are controlled by Src family kinases (SFKs). Furthermore, the authors provide evidences that the traffic-induced SFKs activation is due to ER chaperones leaving from ER and binding to the KDEL receptor through their KDEL motif in Golgi complex. The KDEL-receptor directly binds with Src proteins and triggers phosphorylation cascade on the Golgi responding for the protein trafficking from cis-Golgi to plasma membrane. This signalling reaction activates the intra-Golgi transport machinery and may also enhance Golgi-to-ER recycling. Finally, it was demonstrated that activation of SFKs is required for both pulse and normal intra-Golgi trafficking in mammalian cells. The findings from this study can help us to understand the Golgi-based signalling circuits in dynamic equilibrium during trafficking.




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