Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin (Cell, 2008, 135:272-283)

報告日期: 2009/12/15
報告時間: 15:10/16:00
報告學生: 鄭昭旻
講評老師: 陳炳焜

Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin

Fei Li,Yang Shi, W. Zacheus Cande, et al. Cell 135, 272–283, October 17, 2008


Speaker: Chao-Min, Cheng

Commentator: Ben-Kuen, Chen

Date/Time: 2009/12/15 15:10-16:00



   Chromosomal processes are affected by many posttranslational modifications of histones; one of these important modifications is methylation. It is known that H3K4 hypermethylation and H3K9 hypomethylation are related to euchromatin, while H3K4 hypomethylation and H3K9 hypermethylation are the heterochromatin symbols. In this study the author focused on the histone demethylase, Little imaginal discs2 (Lid2), to examine whether it can establish a proper methylation pattern by coordinating with other enzymes. Results from a series of immunoprecipitation and immunostaining experiments indicated that wt-Lid2 are essential for chromosome segregation and heterochromatin silencing. Lid2 activity depends on JmjC-domain, iron-binding site and PHD domain. The RNAi protein immunoprecipitation data revealed that Lid2 plays a critical role in the RNA interference. The most important evidence was that Lid2 could recruit H3K9 demethylase Lsd1 and H3K4 methyltransferase Set1 to regulate chromatin silence. In summary, this study showed that Lid2 critically correlate with chromatin regulation and modulate gene expression directly or indirectly.  



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