Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project (Nature, 2007, 447:799-816)

報告日期: 2009/01/06
報告時間: 16:00/16:50
報告學生: 陳疇丞(英文報告)
講評老師: 孫孝芳
附件下載:

http://basicmed.med.ncku.edu.tw/admin/up_img/980106-2.pdf

Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project

 

Nature. 2007 June 14; 447(7146): 799–816.

 

Speaker: C. C. Chen (陳疇丞)

Commentator: 孫孝芳 教授

Time: 2009. 1. 6 (PM: 4:00~4:50)

 

Abstract:

ENCODE pilot project collects the high-throughput data and uses computational analysis to build database. The author offers advanced collective knowledge covering four parts of human genome function. First of all, the author detect human genome that is pervasively transcribed by providing convince data; it transcribes not only in majority of primary transcript sites but also in other regions including non-protein coding regions and extensively overlapping one another. Second, the author offers the new understanding about transcription start sites; the new understanding are include their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. Third, the author shows the data to interpret the chromatin structure that has interrelationship with DNA replication and regulation of transcription. Finally, the author offers functional landscape of human genome; it is established through integrating new information by intra- and inter- species sequence comparison and then through yielding novel mechanistic and new insights of human evolution. In conclusion, this study supplies a more comprehensive characterization of human genome function; it also provides clues and unveils the possibility of revolution; however, it still has many questions about the process of revolution by gene selection of genes that we can investigate.

 

References: 

1.    Encode Project Consortium, et al. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature. 2007;447(7146):799-816

2.    Kapranov P, et al. Examples of the complex architecture of the human transcriptome revealed by RACE and high-density tiling arrays. Genome Res. 2005;15:987–97.