Nano particle-mediated cellular response is size-dependent (Nat Nanotechnol, 2008, 3:145-150)

報告日期: 2009/04/07
報告時間: 17:10/18:00
報告學生: 黃彥彰
講評老師: 黎煥耀
附件下載:

http://basicmed.med.ncku.edu.tw/admin/up_img/980407-3.pdf

Nanoparticle-mediated cellular response

is size-dependent

nature nanotechnology | VOL 3 |MARCH 2008 |

 

Speaker: 黃彥彰

Commentator: 黎煥耀 老師

Date: 2009/04/07

Time: 17:10~18:00

 

Abstract

  Nanotechnology refers to research and technology development at the atomic, molecular, and macromolecular scale, leading to the controlled manipulation and study of structures and devices with length scales in the 1- to 100-nanometers range. Recent observations in biological systems suggest that the physical parameters of nanoparticles can affect their nonspecific uptake in cells, with potential to induce cellular responses. In spite of what has been achieved so far, a complete understanding of how cells interact with nanostructures of well-defined sizes, at the molecular

level, remains poorly understood. Here we show that gold and silver nanoparticles coated with antibodies can regulate the process of membrane receptor internalization. The binding and activation of membrane receptors and subsequent protein expression strongly depend on nanoparticle size. Although all nanoparticles within the 2–100 nm size range were found to alter signalling processes essential for basic cell functions (including cell death), 40- and 50-nm nanoparticles demonstrated the greatest effect. This concept has significant implications in understanding the interactions of nanostructures with biological systems and assisting in the design of intelligent nanodevices, with great

potential for the development of novel molecular-based diagnostics and therapeutics.

 

Reference

Elghanian, R., Storhoff, J. J., Mucic, R. C., Letsinger, R. L. & Mirkin, C. A. Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science 227, 1078–1081 (1997).