Identification of CD15 as a marker for tumor-propagating cells in a mouse model of medulloblastoma (Cancer Cell, 2009, 15:135-147)

報告日期: 2009/04/24
報告時間: 16:00/16:50
報告學生: 楊明哲(英文報告)
講評老師: 賴明德
附件下載:

http://basicmed.med.ncku.edu.tw/admin/up_img/980424-2.pdf

Identification of CD15 as a Marker for Tumor-Propagating Cells in a Mouse Model of Medulloblastoma

Cancer Cell 15, 135–147, February 2009

 

SpeakerYoung Ming-Jer 楊明哲

CommentatorProf. Lai Ming-Derg 賴明德教授

Date : 14 April, 2009 (16:10~17:00)

Place : Room 602

 

Abstract:

 

Theory of cancer stem cells refers to a subset of tumor cells with the ability to self-renewal and differentiation. Only those tumor cells, there are these two properties is the so-called cancer stem cells or the tumor propagating cells (TPCs). The idea was first demonstrated in the study of leukemia, only a specific surface antigen profiles can induce leukemia when grafted into immune-deficient mice. In recent years, solid tumors were studied using similar technology. Human tumor evidence of cancer stem cells has been published, including breast tumor, brain, pancreas, head and neck, and colon.

Human brain tumor cells in the expression of stem cell marker CD133 have been involved in TPCs. Although tumor-propagating cells (TPCs) have been described in human brain tumors, such cells have not been identified in mouse models of the disease. Mouse model to investigate the TPCs is critical because it allows the study of the origin and development of its experimental operation, and for these cells in a species matching environment.

Here the authors show that the tumor model of medulloblastoma, the Patched mutant mice are propagated not by CD133+ cells but by cells expressing the progenitor markers Math1 and CD15. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastoma and tumor gene expression similar to those found in CD15 + cells in mice with a poor prognosis. Therefore, CD15 may be an important sign of TPCs in human medulloblastoma.

In short, this study was to determine a population of TPCs in a mouse model of medulloblastoma. The fact that these cells express markers of progenitors and cannot form neurospheres indicates that a stem-like phenotype is not a necessary feature of TPCs. Identification of TPCs in a mouse model of human medulloblastoma will allow us to study the mechanism of tumor promotion and test their methods against them in vivo.

 

References:

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3.   Sheila K. Singh, Cynthia Hawkins, Ian D. Clarke, Jeremy A. Squire, Jane Bayani, Takuichiro Hide,R.Mark Henkelman, Michael D. Cusimano , Peter B. Dirks. Identification of human brain tumour initiating cells. Nature. 438;18, 396-401, 2004.